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PromoCell human aortic endothelial cells haoec
Figure 1. Expression of ephrinB2 in adult human aorta (A), saphenous vein (A), fetal umbilical artery (B), and fetal umbilical vein (B) in vivo (left) and in vitro (right). Sections from human aorta <t>(HAoEC)</t> (A), human saphenous vein (HSaVEC) (A), umbili- cal artery (HUAEC) (B), and umbilical vein (HUVEC) (B) were an- alyzed histochemically for the expression of CD34 (IHC-CD34) and by in situ hybridization for ephrinB2 (ISH-EB2). Correspond- ingly, monolayer of cultured human aortic EC (A), human saphe- nous vein EC (A), umbilical artery EC (B), and umbilical vein EC (B) were pelleted, embedded, and sectioned for the analysis of immunohistochemical CD31 detection (IHC-CD31) and in situ hybridization for ephrinB2 (ISH-EB2). Human aortic EC (HAoEC) in vivo are uniformly ephrinB2-positive (A, green arrows). In con- trast, human saphenous EC (HSaVEC) in vivo are uniformly ephrinB2-negative (A, red arrows). ECs in the umbilical artery and in the umbilical vein are both ephrinB2-positive (B, green arrows). Cultured ECs are all partially ephrinB2-positive, irre- spective of their arterial or venous origin (A and B, green arrow- heads). Scale bar, 100 m.
Human Aortic Endothelial Cells Haoec, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+12271/Human+Aortic+Endothelial+Cells/10__1161_slash_01__atv__0000200081__42064__e7-152-12-34
Average 96 stars, based on 1 article reviews
human aortic endothelial cells haoec - by Bioz Stars, 2026-11
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Figure 1. Expression of ephrinB2 in adult human aorta (A), saphenous vein (A), fetal umbilical artery (B), and fetal umbilical vein (B) in vivo (left) and in vitro (right). Sections from human aorta (HAoEC) (A), human saphenous vein (HSaVEC) (A), umbili- cal artery (HUAEC) (B), and umbilical vein (HUVEC) (B) were an- alyzed histochemically for the expression of CD34 (IHC-CD34) and by in situ hybridization for ephrinB2 (ISH-EB2). Correspond- ingly, monolayer of cultured human aortic EC (A), human saphe- nous vein EC (A), umbilical artery EC (B), and umbilical vein EC (B) were pelleted, embedded, and sectioned for the analysis of immunohistochemical CD31 detection (IHC-CD31) and in situ hybridization for ephrinB2 (ISH-EB2). Human aortic EC (HAoEC) in vivo are uniformly ephrinB2-positive (A, green arrows). In con- trast, human saphenous EC (HSaVEC) in vivo are uniformly ephrinB2-negative (A, red arrows). ECs in the umbilical artery and in the umbilical vein are both ephrinB2-positive (B, green arrows). Cultured ECs are all partially ephrinB2-positive, irre- spective of their arterial or venous origin (A and B, green arrow- heads). Scale bar, 100 m.

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: Endothelial EphrinB2 Is Controlled by Microenvironmental Determinants and Associates Context-Dependently With CD31

doi: 10.1161/01.atv.0000200081.42064.e7

Figure Lengend Snippet: Figure 1. Expression of ephrinB2 in adult human aorta (A), saphenous vein (A), fetal umbilical artery (B), and fetal umbilical vein (B) in vivo (left) and in vitro (right). Sections from human aorta (HAoEC) (A), human saphenous vein (HSaVEC) (A), umbili- cal artery (HUAEC) (B), and umbilical vein (HUVEC) (B) were an- alyzed histochemically for the expression of CD34 (IHC-CD34) and by in situ hybridization for ephrinB2 (ISH-EB2). Correspond- ingly, monolayer of cultured human aortic EC (A), human saphe- nous vein EC (A), umbilical artery EC (B), and umbilical vein EC (B) were pelleted, embedded, and sectioned for the analysis of immunohistochemical CD31 detection (IHC-CD31) and in situ hybridization for ephrinB2 (ISH-EB2). Human aortic EC (HAoEC) in vivo are uniformly ephrinB2-positive (A, green arrows). In con- trast, human saphenous EC (HSaVEC) in vivo are uniformly ephrinB2-negative (A, red arrows). ECs in the umbilical artery and in the umbilical vein are both ephrinB2-positive (B, green arrows). Cultured ECs are all partially ephrinB2-positive, irre- spective of their arterial or venous origin (A and B, green arrow- heads). Scale bar, 100 m.

Article Snippet: Human umbilical vein endothelial cells (HUVEC), human umbilical artery endothelial cells (HUAEC), human aortic endothelial cells (HAoEC), human saphenous vein endothelial cells (HSaVEC) and human umbilical artery smooth muscle cells (HUASMC) were purchased from Promocell (Heidelberg, Germany) and cultured at 37°C using endothelial cell growth media (ECGM) and endothelial cell population specific growth supplements according to manufacturers instructions.

Techniques: Expressing, In Vivo, In Vitro, In Situ Hybridization, Cell Culture, Immunohistochemical staining